Invention Grant
- Patent Title: Methods for microbial DNA analysis
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Application No.: US16816481Application Date: 2020-03-12
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Publication No.: US11319582B2Publication Date: 2022-05-03
- Inventor: Michael Edward Hogan , Benjamin Alan Katchman , Candy Mavis Rivas , Yasmine Eve Baca
- Applicant: Michael Edward Hogan , Benjamin Alan Katchman , Candy Mavis Rivas , Yasmine Eve Baca
- Applicant Address: US NJ Stony Brook; US AZ Tucson; US AZ Tucson; US AZ San Tan Valley
- Assignee: Michael Edward Hogan,Benjamin Alan Katchman,Candy Mavis Rivas,Yasmine Eve Baca
- Current Assignee: Michael Edward Hogan,Benjamin Alan Katchman,Candy Mavis Rivas,Yasmine Eve Baca
- Current Assignee Address: US NJ Stony Brook; US AZ Tucson; US AZ Tucson; US AZ San Tan Valley
- Agent Benjamin Aaron Adler
- Main IPC: C12Q1/6851
- IPC: C12Q1/6851

Abstract:
Provided herein are methods for isolating nucleic acids from intact cells in a sample of intact cells, contamination dead cells, cell debris, and biofilm using two separation steps, either by centrifugation or filtration, performed in sequentially. Also provided is a method for isolating nucleic acids from intact cells using a first separation step followed by treatment with a nuclease and then a second separating step. Provided herein is a related method for isolating DNA from intact cells using a nuclease that produces DNA cuts on double stranded DNA, followed by a second separating step.
Public/Granted literature
- US20210017590A1 Methods for Microbial DNA Analysis Public/Granted day:2021-01-21
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