A hydrodynamic power generation assembly and method of use therefor for generating electrical power from the combination of kinetic energy, hydrostatic energy, and turbulent energy of water. The power generation assembly comprises a water accelerator assembly comprising a support structure which is at least partially buoyant and a baffle panel member (or an array of baffle panel members) having an opening, inter-panel spacing, or flow passageway around the baffle panel(s). A hydropower converter is supported from, by, or on the support structure and is operatively coupled to a generator. The hydropower converter is positioned behind baffle assembly. Water flowing through or around the baffle assembly has an increased velocity relative the ambient current and therefore is capable of generating more power relative to the ambient water where power generation assembly is deployed. Particular types of hydropower converters suitable for use with the invention are turbines and water wheels.