Disclosed are devices and methods using the devices for collecting biological and other specimens or substances from surfaces being interrogated for such contamination. Particular device aspects comprise a handle, with a frame at one end receptive to insertion and removal of a sampling medium (collecting member) intended for wiping against a surface, with the sampling member being held in place by passive projections from the frame without the need for the use of glue or articulating or moving parts. The Frame and/or the collecting member may also comprise at least one attached or integral scraping member or surface for breaking biofilms and thus making the microbes or other substances more available to being sampled by the collecting member.