Invention Grant
- Patent Title: Combination of reversible and irreversible cell labeling for analizing receptor-ligand koff rate
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Application No.: US16314126Application Date: 2017-06-27
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Publication No.: US11320431B2Publication Date: 2022-05-03
- Inventor: Magdalena Nauerth , Dirk Busch , Lothar Germeroth
- Applicant: TECHNISCHE UNIVERSITAET MUENCHEN , JUNO THERAPEUTICS GMBH
- Applicant Address: DE Munich; DE Munich
- Assignee: TECHNISCHE UNIVERSITAET MUENCHEN,JUNO THERAPEUTICS GMBH
- Current Assignee: TECHNISCHE UNIVERSITAET MUENCHEN,JUNO THERAPEUTICS GMBH
- Current Assignee Address: DE Munich; DE Munich
- Agency: Acuity Law Group, PC
- Agent Michael A. Whittaker
- Priority: EP16176537 20160628
- International Application: PCT/EP2017/065755 WO 20170627
- International Announcement: WO2018/001985 WO 20180104
- Main IPC: G01N33/557
- IPC: G01N33/557 ; G01N33/566 ; G01N33/543 ; G01N33/50 ; C07K14/74 ; C12Q1/04 ; C12N5/0783
Abstract:
The invention relates to a method of determining the dissociation rate constant (koff) of a receptor molecule R on a target cell using a combination of reversible and irreversible cell labeling. The invention further relates to a cell comprising such a receptor molecule R, wherein the cell has bound to it such a combination of cell labeling. The invention further relates to a kit and an apparatus useful in performing the methods of the invention. The invention further relates to a method of isolation a high-avidity T cell.
Public/Granted literature
- US20190227061A1 COMBINATION OF REVERSIBLE AND IRREVERSIBLE CELL LABELING FOR ANALIZING RECEPTOR-LIGAND KOFF RATE Public/Granted day:2019-07-25
Information query
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