An embodiment of the present invention discloses a display substrate and a display panel. The display substrate includes a plurality of first display portions each of which is disposed in a gap between adjacent two of first limiting portions; a plurality of second display portions each of which is disposed in a gap between adjacent two of second limiting portions. The gap between the adjacent two first limiting portions is greater than the gap between the adjacent two second limiting portions. A difference between a height of a top of each of the second display portions and a height of a top of each of the first display portions is less than a predetermined difference.