The present disclosure discloses a multicolor barcode. The multicolor barcode includes a start region (1), a data region (2), and a check region (3). The start region (1), the data region (2), and the check region (3) are arranged in sequence. The start region (1) includes a start color block that indicates a start position of the multicolor barcode, the data region (2) includes a data color block that indicates encoded data of the multicolor barcode, and the check region (3) includes a check color block that indicates an end position of the multicolor barcode. The start color block includes a first color block and a second color block, the first color block is located at the start position, and the second color block is a white color block. The present disclosure further provides a color calibration method for a multicolor barcode, and a computer-readable storage medium.