A quantum dot film, a quantum dot light-emitting assembly and a display device are provided. The quantum dot film includes: a quantum dot layer; and a conductive layer arranged on at least a side of the quantum dot layer along a thickness direction, and the conductive layer includes nano-sized metal particles, and at least a portion of the nano-sized metal particles are configured to generate a surface plasmon resonance under electromagnetic radiation. The luminescence efficiency and intensity of the quantum dot layer can be effectively improved by arranging the conductive layer on at least a side of the quantum dot layer.