A digital-to-analog conversion circuit, a digital-to-analog conversion method, and a display device are provided. The digital-to-analog conversion circuit includes a first digital-to-analog conversion sub-circuit and a second digital-to-analog conversion sub-circuit. The second digital-to-analog conversion sub-circuit includes least-significant-bit voltage selection modules whose quantity is a, a weighed summation operational amplifier, switching control modules whose quantity is a and energy storage modules whose quantity is a. The weighted summation operational amplifier includes a reverse-phase input end, an operational amplification output end, and same-phase input ends whose quantity is a. The reverse-phase input end is connected to the operational amplification output end, and a is an integer greater than 1. The weighted summation operational amplifier is configured to perform weighted summation on voltages inputted by the a same-phase input ends at a digital-to-analog conversion stage to acquire an analog voltage, and output the analog voltage via the operational amplification output end.