A latex glove inflation assembly includes a housing that is fluidly coupled to a vacuum source for purging the housing of air. The housing has a pair of glove apertures extending into an interior of the housing. A plurality of engagements is each distributed around a respective one of the glove apertures. Each of the engagements engages a wrist opening on a respective pair of latex gloves when the engagements are turned on. The latex gloves are inflated when the housing is purged of air thereby facilitating a user to insert their hands into the gloves. Each of the engagements releases the latex gloves when the engagements are turned off. A release is coupled to the housing and the release facilitates air to fill the housing when the release is turned on thereby facilitating each of the latex gloves to deflate around the user's hands.