The invention relates to a cutting tool with a holder, a cutting insert and a screw, wherein the holder has at least one seat with a base, a first side wall and a second side wall, wherein in a plane parallel to the base the first side wall extends in a first direction and the second side wall includes an acute angle (C) with the first side wall, wherein the base has a threaded hole for receiving the screw, wherein the cutting insert has a cut-out for the screw, wherein the cutting insert has a base contact surface, a first positioning surface and a second positioning surface, and wherein in a locked position the base contact surface, the first positioning surface and the second positioning surface of the cutting insert contact the base, the first side wall and the second side wall of the at least one seat, respectively, wherein the second side wall is offset from the threaded hole in the first direction towards a front end of the seat, and in that a screw head of the screw and the cut-out of the cutting insert are provided with complementary conical surfaces, such that when tightening the screw the cutting insert is forced with the first positioning surface and the second positioning surface against the first side wall and the second side wall, respectively.