The present application discloses a display substrate. The display substrate includes a base substrate; a color filter on the base substrate; and an overcoat layer on a side of the color filter distal to the base substrate. The overcoat layer includes a first sublayer and a second sublayer, the second sublayer on a side of the first sublayer distal to the base substrate. The first sublayer includes a first polymer material. The second sublayer includes a second polymer material different from the first polymer material.