A lane line determination method and apparatus, a lane line positioning accuracy evaluation method and apparatus, a device and a storage medium are provided, which are related to a field of image processing, and particularly to fields of autonomous driving, intelligent transportation, computer vision and the like. The specific implementation is: determining a line in a received road image; screening pixels forming the line and determining pixels forming a lane line; and fitting the pixels forming the lane line to obtain the lane line. According to the technology of the present disclosure, the disadvantages of manual labeling can be overcome, and the lane line in the image collected by image acquisition device can be automatically recognized using an image recognition method, thereby improving the automation degree of lane labeling.