The present disclosure provides an ultrasonic fingerprint sensor, a display substrate, a driving method thereof and a display device. The ultrasonic fingerprint sensor includes: a base substrate; a driving structure provided on the base substrate and configured to transmit an ultrasonic signal; a receiving structure provided on the base substrate and configured to receive the ultrasonic signal reflected by a finger, and convert the ultrasonic signal into an electrical signal for determining fingerprint information, the driving structure and the receiving structure are provided in a same plane. Since the driving structure and the receiving structure of the ultrasonic fingerprint sensor of the present disclosure are in a same plane, the driving structure can be closer to the touch body (for example, a finger), which is advantageous for the ultrasonic waves generated by the driving structure to reach the finger to improve the touch accuracy.