The present disclosure relates to a pixel compensation method and device, a storage medium, and a display screen, and belongs to the field of display technologies. The method includes: sensing a plurality of subpixels in a first target grayscale of a display screen by using a plurality of photosensitive units, to obtain an actual luminance value of each subpixel; determining a theoretical luminance value of each subpixel in the first target grayscale based on a compensation sensing model, where the compensation sensing model is used to record a correspondence between target grayscales and theoretical pixel data, and the theoretical pixel data includes a reference luminance value of each subpixel; and performing pixel compensation on each subpixel based on the actual luminance value of each subpixel and the theoretical luminance value of each subpixel.