An endophytic fungus from gingko, that is, Fusarium proliferatum DZHQ1 having antitumor activity and antibacterial activity, is isolated from the ginkgo bark. The species of the strain is determined by the combination of colonial morphology and 18 sRNA sequencing, and then the anti-cervical cancer activity of a crude extract of the strain is detected by MTT. Finally, a secondary metabolite of the strain with an inhibition rate of more than 50% is isolated by semi-preparative HPLC, which promotes the further screening of individual compounds with anti-tumor activity. Moreover, the metabolite of the endophytic fungus from gingko shows a more pronounced inhibition in the detection of activity against E. coli and/or S. aureus, and has potential use in the preparation of new antibacterial products.