Invention Grant
- Patent Title: Nucleic acid melting analysis with saturation dyes
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Application No.: US16844675Application Date: 2020-04-09
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Publication No.: US11332779B2Publication Date: 2022-05-17
- Inventor: Luming Zhou , Carl T. Wittwer , Philip Seth Bernard , Virginie Dujols
- Applicant: University of Utah Research Foundation , BioFire Defense, LLC
- Applicant Address: US UT Salt Lake City; US UT Salt Lake City
- Assignee: University of Utah Research Foundation,BioFire Defense, LLC
- Current Assignee: University of Utah Research Foundation,BioFire Defense, LLC
- Current Assignee Address: US UT Salt Lake City; US UT Salt Lake City
- Agency: Workman Nydegger
- Main IPC: C12Q1/6851
- IPC: C12Q1/6851 ; C12Q1/6883 ; C09B23/04 ; C12Q1/6813

Abstract:
Methods are provided for nucleic acid analysis wherein a target nucleic acid is mixed with a dsDNA binding dye to form a mixture. Optionally, an unlabeled probe is included in the mixture. A melting curve is generated for the target nucleic acid by measuring fluorescence from the dsDNA binding dye as the mixture is heated. Dyes for use in nucleic acid analysis and methods for making dyes are also provided.
Public/Granted literature
- US20200248247A1 Nucleic Acid Melting Analysis with Saturation Dyes Public/Granted day:2020-08-06
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