Invention Grant
- Patent Title: SO3 chromatography for use in a method for virus purification
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Application No.: US16762119Application Date: 2018-11-08
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Publication No.: US11339377B2Publication Date: 2022-05-24
- Inventor: Markus Wolschek , Manfred Reiter , Ales Strancar , Mojca Tajnik Sbaizero
- Applicant: Blue Sky Vaccines GmbH , BIA Separations D.O.O
- Applicant Address: AT Vienna; SI Ljubljana
- Assignee: Blue Sky Vaccines GmbH,BIA Separations D.O.O
- Current Assignee: Blue Sky Vaccines GmbH,BIA Separations D.O.O
- Current Assignee Address: AT Vienna; SI Ljubljana
- Agency: Loza & Loza, LLP
- Agent Michael F. Fedrick
- Priority: EP17200517 20171108
- International Application: PCT/EP2018/080571 WO 20181108
- International Announcement: WO2019/092084 WO 20190516
- Main IPC: C07K14/00
- IPC: C07K14/00 ; A61K38/00 ; C07H21/04 ; C07K14/765 ; C12Q1/68 ; C12N7/00
Abstract:
The present invention refers to a process for purifying virus particles from cell culture, comprising the steps of subjecting the cell culture to centrifugation to get a supernatant fraction of virus particles, incubating said fraction with a nuclease, diluting the fraction with low conductivity buffer, subjecting said diluted fraction to a SO3 chromatography step, performing a washing step with low conductivity buffer, and eluting virus particles.
Public/Granted literature
- US20200332264A1 SO3 CHROMATOGRAPHY FOR USE IN A METHOD FOR VIRUS PURIFICATION Public/Granted day:2020-10-22
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