A pixel arrangement structure, a display substrate, a display device, and a mask plate group. The pixel arrangement structure includes multiple minimum repeat units arranged in an array; each minimum repeat unit includes a first color sub-pixel, a second color sub-pixel, and a third color sub-pixel; a first connecting line between the centers of the first color sub-pixel and the second color sub-pixel extends along a first direction, and a second connecting line between the centers of the first color sub-pixel and the third color sub-pixel extends along a second direction; the orthographic projection of the first color sub-pixel on a first straight line falls into the orthographic projection of the third color sub-pixel on the first straight line, and the orthographic projection of the first color sub-pixel on a second straight line falls into the orthographic projection of the second color sub-pixel on the second straight line.