The present disclosure relates to a pre-5th-Generation (5G) or 5G communication system to be provided for supporting higher data rates Beyond 4th-Generation (4G) communication system such as Long Term Evolution (LTE). A method of operating a terminal in a wireless communication system includes receiving control information for grant-free communication from a base station, generating a reference signal by using one sequence among sequences included in sequence set information for the reference signal included in the control information, and transmitting the reference signal and a data signal to the base station by using grant-free resource region information included in the control information. The one sequence may be used to allow the base station to identify the terminal.