The present invention relates to a duster cloth for a cleaning robot and a cleaning robot using the duster cloth. The duster cloth comprises a detachable connection part (21) close to a base of the robot, a wiping cloth (22) close to a surface to be cleaned, and an elastic sealing layer located between the detachable connection part (21) and the wiping cloth (22). The elastic sealing layer is attached to both the detachable connection part (21) and the wiping cloth (22). The elastic sealing layer is made from a foamed EPDM material (23), an integral skin sponge (33) or a common sponge (43), wherein a sealing film (24) is arranged on at least one side of the common sponge (43). The detachable connection part (21) may be a Velcro. With the structure of the elastic sealing layer between the detachable connection part and the wiping cloth, the duster cloth has good elasticity, and the air-tightness of the negative pressure chamber of the cleaning robot is greatly improved.