A decolonization drug, a preparation method thereof and an application thereof. The decolonization drug is non-antibiotic, simple and convenient to use, and can be used systemically, and has more types of sterilization; The decolonization drug involved in the invention contains added special components, which can improve the bactericidal performance of the drug through the activator effect; The decolonization drug is suitable for use in adults and sensitive populations, including the elderly, pregnant women, infants, children, etc.; especially as a mucosal drug for nasal mucosa, oral mucosa, ocular mucosa, abdominal mucosa, genital mucosa, etc., and can also be used as a bactericidal drug in other medical fields, including diabetic foot, burns, surgical site infections (SSIs), etc.