A server chassis, configured to detachably fasten a PCB, includes a case, an expansion frame, and a fastening assembly. The fastening assembly includes a slidable component, a guiding component disposed through the slidable component and fixed to the case, a movable pin movably disposed through the slidable component, and a fastening pin fixed to the slidable component. When the slidable component is at a fastening position, the movable pin is movable between inserted and extracted positions. When the movable pin is at the inserted position, the movable pin is disposed through the slidable component and the case, such that the slidable component is positioned at the fastening position, and the fastening pin is disposed through the PCB and the expansion frame. When the movable pin is at the extracted position, the movable pin is disengaged from the case, such that the slidable component is slidable to a releasing position.