A fingerprint sensing device and an operation method thereof are provided. The fingerprint sensing device includes a fingerprint sensor, a driver integrated chip, and a fingerprint sensing chip. The fingerprint sensing chip is coupled to the fingerprint sensor, and is coupled to the driver integrated chip via a general purpose input/output (GPIO) interface. The fingerprint sensing chip provides a control signal to the driver integrated chip via the GPIO interface to control the driver integrated chip according to the control signal to disable touch sensing during a fingerprint sensing period. The fingerprint sensing chip drives the fingerprint sensor to obtain a fingerprint image during the fingerprint sensing period.