Disclosed is a system for separating and coupling a top nozzle of a nuclear fuel assembly. There is provided a lock insert configured to support the top nozzle of the nuclear fuel assembly by being coupled to a guide hole provided in a flow channel plate of the top nozzle, the lock insert including: a body in a hollow shape; and an insertion part provided on a top portion of the body and inserted into the guide hole, wherein a circumference of the insertion part is variable in size, thereby being capable of being inserted into the guide hole. Accordingly, disassembly and reassembly of the top nozzle of the nuclear fuel assembly and the lock insert are simplified, thereby simplifying and reducing the number of processes involved therein. Accordingly, the system is effective for maintenance and repair of the nuclear fuel assembly.