Endoscopic instruments, such as endoscopes, and devices and methods for cleaning endoscopic instruments are provided. A cleaning device for use with an endoscopic instrument comprises an elongate member configured for advancement through an internal lumen within the endoscopic instrument and a cleaning member removably coupled to a portion of the elongate member. The cleaning element comprises a variable pressure region shaped and configured to increase the hydrodynamic fluid friction force and fluid pressure force of a cleaner or detergent against the wall of the internal lumen of the endoscope to more effectively clean all internal surfaces of an endoscopic instrument, including crevasses, scratches or other irregularities, without further damaging these surfaces.