A pixel circuit, a pixel driving method and a display device are provided in the present disclosure. The pixel circuit includes a light-emitting element, a pixel driving circuit, an on-off control circuit and a light-emission time control circuit. A control terminal of the on-off control circuit is electrically coupled to the light-emission time control circuit, and the on-off control circuit is configured to control a driving current output terminal to be coupled to, or decoupled from, the light-emitting element under the control of a potential at the control terminal of the on-off control circuit. The light-emission time control circuit is configured to control an nth light-emission time control signal output terminal to be coupled to, or decoupled from, the control terminal of the on-off control circuit in accordance with an nth light-emission control data voltage under the control of a second gate driving signal. N is an integer larger than 1, and n is a positive integer smaller than or equal to N.