The present invention provides a quantum dot display panel and a manufacturing method thereof. The present invention includes a pixel definition layer on a surface of a color film substrate, a quantum dot photoconversion film located on pixel definition regions of the pixel definition layer, and a blue light display device located under the quantum dot photoconversion film. An uplift layer and a light-shielding layer are arranged in the pixel definition layer to ensure that a thickness of the pixel definition layer is greater than or equal to 6 μm, which is beneficial to uniform curing of quantum dots in the quantum dot photoconversion film.