Single-use devices configured to sanitize accessible surfaces of luer access devices (e.g., needleless medical valves) at risk of contamination with infectious agents are described, as are methods for making and using such devices. In particularly preferred embodiments, such devices include a container (e.g., laminated foil pouches or packets) that contain a sanitizing component that includes a substrate with a cleaning port or recess, a cleansing matrix associated with the substrate, and a sanitizing reagent dispersed in the cleansing matrix.