Invention Grant
- Patent Title: Method for producing retinal tissue
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Application No.: US16095339Application Date: 2017-04-21
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Publication No.: US11371016B2Publication Date: 2022-06-28
- Inventor: Atsushi Kuwahara , Suguru Yamasaki , Yoshiki Sasai , Masayo Takahashi
- Applicant: SUMITOMO PHARMA CO., LTD. , RIKEN , SUMITOMO CHEMICAL COMPANY, LIMITED
- Applicant Address: JP Osaka; JP Wako; JP Tokyo
- Assignee: SUMITOMO PHARMA CO., LTD.,RIKEN,SUMITOMO CHEMICAL COMPANY, LIMITED
- Current Assignee: SUMITOMO PHARMA CO., LTD.,RIKEN,SUMITOMO CHEMICAL COMPANY, LIMITED
- Current Assignee Address: JP Osaka; JP Wako; JP Tokyo
- Agency: Leydig, Voit & Mayer, Ltd.
- Priority: JPJP2016-086602 20160422
- International Application: PCT/JP2017/016120 WO 20170421
- International Announcement: WO2017/183732 WO 20171026
- Main IPC: A61K35/30
- IPC: A61K35/30 ; C07K14/71 ; A61P27/02 ; C12N5/074 ; G01N33/50 ; A61L27/38 ; A61L27/54 ; C12N5/0793 ; A61P27/06 ; A61P9/10 ; G01N33/15

Abstract:
The present invention provides a method for producing a retinal cell or a retinal tissue, including the following steps (1)-(3): (1) a first step of culturing pluripotent stem cells in the absence of feeder cells in a medium containing 1) a TGFβ family signal transduction pathway inhibiting substance and/or a Sonic hedgehog signal transduction pathway activating substance, and 2) a factor for maintaining undifferentiated state, (2) a second step of culturing the cells obtained in the first step in suspension in a medium containing a Wnt signal transduction pathway inhibiting substance to form a cell aggregate, and (3) a third step of culturing the aggregate obtained in the second step in suspension in the presence or absence of a Wnt signal transduction pathway inhibiting substance in a medium containing a BMP signal transduction pathway activating substance to obtain an aggregate containing a retinal cell or a retinal tissue.
Public/Granted literature
- US20190127690A1 METHOD FOR PRODUCING RETINAL TISSUE Public/Granted day:2019-05-02
Information query
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