Invention Grant
- Patent Title: Recombinant polymerases with increased phototolerance
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Application No.: US17025722Application Date: 2020-09-18
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Publication No.: US11377644B2Publication Date: 2022-07-05
- Inventor: Satwik Kamtekar , Lei Jia , Erik Miller
- Applicant: Pacific Biosciences of California, Inc.
- Applicant Address: US CA Menlo Park
- Assignee: Pacific Biosciences of California, Inc.
- Current Assignee: Pacific Biosciences of California, Inc.
- Current Assignee Address: US CA Menlo Park
- Agent Monicia Elrod-Erickson
- Main IPC: C12N9/12
- IPC: C12N9/12 ; C12P19/34 ; C12Q1/6869

Abstract:
Provided are compositions comprising recombinant DNA polymerases that include amino acid substitutions, insertions, deletions, and/or exogenous features that confer modified properties upon the polymerase for enhanced single molecule sequencing. Such properties include increased resistance to photodamage, and can also include enhanced metal ion coordination, reduced exonuclease activity, reduced reaction rates at one or more steps of the polymerase kinetic cycle, decreased branching fraction, altered cofactor selectivity, increased yield, increased thermostability, increased accuracy, increased speed, increased readlength, and the like. Also provided are nucleic acids which encode the polymerases with the aforementioned phenotypes, as well as methods of using such polymerases to make a DNA or to sequence a DNA template.
Public/Granted literature
- US20210155908A1 RECOMBINANT POLYMERASES WITH INCREASED PHOTOTOLERANCE Public/Granted day:2021-05-27
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