Invention Grant
- Patent Title: Amylase mutant having high specific activity and thermal stability, gene of mutant, and applications thereof
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Application No.: US16982628Application Date: 2019-03-21
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Publication No.: US11377647B2Publication Date: 2022-07-05
- Inventor: Bin Yao , Huiying Luo , Jin Qiu , Tao Tu , Huoqing Huang , Yuan Wang , Yaru Wang , Yingguo Bai , XiaoYun Su , Kun Meng
- Applicant: INSTITUTE OF ANIMAL SCIENCE OF CHINESE ACADEMY OF AGRICULTURAL SCIENCES
- Applicant Address: CN Beijing
- Assignee: INSTITUTE OF ANIMAL SCIENCE OF CHINESE ACADEMY OF AGRICULTURAL SCIENCES
- Current Assignee: INSTITUTE OF ANIMAL SCIENCE OF CHINESE ACADEMY OF AGRICULTURAL SCIENCES
- Current Assignee Address: CN Beijing
- Agency: Patshegen IP LLC
- Agent Moshe Pinchas
- Priority: CN201810235559.0 20180321
- International Application: PCT/CN2019/079000 WO 20190321
- International Announcement: WO2019/179485 WO 20190926
- Main IPC: C12N9/28
- IPC: C12N9/28 ; C12N1/20 ; C12N15/75
Abstract:
The present invention relates to the field of agriculture biotechnology, specially relates to an amylase mutant having high specific activity and thermal stability, gene and use thereof. Said amylase mutant is obtained by performing substitution of S33A/S34E/V35H, and deletion of amino acids at the sites of 178 and 179 of the wild type amylase having amino acid sequence of SEQ ID NO:1, and having improved enzymatic activity and thermal stability than the wild type amylase.
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