A positioning hose structure for a pull-out faucet contains: a body, a supply pipe, a fitting sleeve, a flexible hose, a guide element, a slide member, and a spring. The body includes a locking portion, and the supply pipe includes an extension, an opening, a defining portion, and a screw element. The fitting sleeve includes a hollow connection tube having a through hole, a receiving orifice, a shoulder, multiple protrusions, and multiple guiding slots. The flexible hose includes a joining portion and a water supply head. The guide element includes a fixing orifice having a positioning portion, and the guide element further includes multiple first ribs and a first toothed portion. The slide member includes multiple second ribs, a second toothed portion, and a protruded tab. The spring is accommodated in the receiving orifice and abuts against the shoulder and the protruded tab.