A display substrate, a manufacturing method thereof and a display device. The display substrate includes a working area and a sealant setting area outside of the working area. The display substrate further includes: a base substrate, a first conductive structure on a first side of the base substrate, and a second conductive structure on one side of the first conductive structure away from the base substrate. The first conductive structure and the second conductive structure are in the sealant setting area. The second conductive structure at least includes an inclined part inclined relative to a main surface of the base substrate. The inclined part is configured to allow at least part of light to be exited out directly over the first conductive structure after the light incident into the inclined part from a second side of the base substrate opposite to the first side is reflected by the inclined part.