Systems and methods for eye tracking calibration in a wearable system are described. The wearable system can present three-dimensional (3D) virtual content and allow a user to interact with the 3D virtual content using eye gaze. During an eye tracking calibration, the wearable system can validate that a user is indeed looking at a calibration target while the eye tracking data is acquired. The validation may be performed based on data associated with the user's head pose and vestibulo-ocular reflex.