A method for determining gray scale values of pixels is provided, which includes the following operations: determining gray scale values of pixels to be displayed of a current display picture and a picture type of the current display picture; determining a target refresh rate according to the picture type; determining a target gray scale correction value according to the target refresh rate, and determining target gray scale values according to the target gray scale correction value and the gray scale values, the target refresh rate and the target gray scale correction value are negatively correlated. A display panel and a computer readable storage medium are also provided. The present disclosure slows down the color unevenness of the display panel.