A light valve panel and a manufacturing method thereof, a three-dimensional printing system, and a three-dimensional printing method are disclosed. The light valve panel includes a first light valve array substrate and at least one second light valve array substrate, the first light valve array substrate and the at least one second light valve array substrate are arranged in a stack; the first light valve array substrate includes a plurality of first pixel units arranged in an array, and the second light valve array substrate includes a plurality of second pixel units arranged in an array; and an orthographic projection of at least one of the second pixel units on the first light valve array substrate partially overlaps with at least one of the first pixel units.