Methods, systems, and media for dynamically separating Internet of Things (IoT) devices in a network are provided. In accordance with some embodiments of the disclosed subject matter, a method for dynamically separating IoT devices in a network is provided, the method comprising: detecting a first IoT device in the network; monitoring network communication of the first IoT device; determining device information of the first IoT device based on the monitored network communication; and causing the first IoT device to communicate on a first subnet of a plurality of subnets in the network based on the device information.