Embodiments of the present disclosure provide a preparation delivery assembly including: a first substrate, a second substrate, and at least two needles of different lengths, each of which is a hollow needle having a hollow structure; wherein two side walls are provided between the first substrate and the second substrate to define a first chamber for containing a preparation by the first substrate, the second substrate, and the two side walls; at least one first channel that is in communication with the first chamber is provided in the second substrate in a direction substantially perpendicular to the second substrate; and the needles are arranged on a surface of the second substrate distal to the first substrate, and each of the needles is in communication with the first chamber through the at least one first channel to deliver the preparation.