Invention Grant
- Patent Title: Engineered yeast strains with signal sequence-modified glucoamylase polypeptides and enhanced ethanol production
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Application No.: US16323458Application Date: 2017-08-04
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Publication No.: US11421212B2Publication Date: 2022-08-23
- Inventor: Christopher K. Miller , Gregory Michael Poynter , Amit Vas
- Applicant: CARGILL, INCORPORATED
- Applicant Address: US MN Wayzata
- Assignee: CARGILL, INCORPORATED
- Current Assignee: CARGILL, INCORPORATED
- Current Assignee Address: US MN Wayzata
- International Application: PCT/US2017/045493 WO 20170804
- International Announcement: WO2018/027131 WO 20180208
- Main IPC: C12N9/34
- IPC: C12N9/34 ; C12P7/06 ; C12P13/04 ; C12P17/04 ; C12P19/14

Abstract:
The invention is directed to non-natural yeast able to secrete significant amounts of glucoamylase into a fermentation media. The glucoamylase can promote degradation of starch material generating glucose for fermentation to a desired bioproduct, such as ethanol. The glucoamylase can be provided in the form of a glucoamylase fusion protein having secretion signal that is: derived from at least AA 1-19 of SEQ ID NO: 73, (ii) an amino acid sequence of at least AA 1-19 of SEQ ID NO: 74, (iii) SEQ ID NO: 77 (An aa), (iv) SEQ ID NO: 75 (Sc IV), (v) SEQ ID NO: 76 (Gg LZ), or (vi) SEQ ID NO: 78(Hs SA).
Information query
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