The present disclosure relates generally to the imaging of blood vessels, and in particular, to the detection of one or more borders of a blood vessel. In some embodiments, a medical imaging system is configured to detect a primary border of a blood vessel and one or more alternative borders of the blood vessel. For example, in some embodiments, a medical imaging system includes an imaging device configured to obtain an image of a blood vessel, a processing unit, and a user display. The processing unit can be configured to receive the image of the blood vessel and perform an analysis of the image to identify a primary candidate border and an alternative candidate border of the blood vessel. The primary candidate border and alternative candidate border can represent candidates for a single boundary of the blood vessel.