Provided is a medium evaluation method for evaluating the suitability of a medium in which cell aggregates are cultured in a suspended state, by which an evaluation of whether a medium is adequate for both the cell retention performance and the cell recovery efficiency, the medium evaluation method including dispersing a plurality of particles in a medium, measuring a sedimentation velocity by which the particles settle in the medium, and using the sedimentation velocity thus measured as an index value indicating the suitability of the medium; and also provided are a medium and a culture method.