Gemstone grading method including illuminating the gemstone by light having a plurality of spectral components, each modulated according to a spectrally specific modulation (e.g., of intensity, frequency, phase), collecting the light following interaction with the gemstone, extracting from the collected light a set of intensity values for a respective set of modulation parameter values to provide a transmittance spectrum, and an output indicative of the transmittance spectrum (e.g., color, clarity, fluorescence grade). A complementary system includes a plurality of monochromatic light sources and respective modulators for simultaneously illuminating a gemstone with a modulation spectrally specific for each light source, a detection system for collecting the light following interaction whose signals are fed via a communication system to a data processor configured to extract a set of intensity values for a respective set of modulation parameter values to provide a transmittance spectrum and an output indicative thereof.