A chromosome recognition method based on deep learning includes the following steps: step 1, obtaining an independent chromosome image; step 2, calculating a manual feature of a chromosome; step 3, performing basic image processing on the chromosome; step 4, building a deep learning model; and step 5, predicting a type of the chromosome based on the deep learning model. By adopting a deep learning method, the chromosome recognition method can be used for recognizing the chromosome type accurately and efficiently. Compared with an existing recognition technology, the chromosome recognition method based on deep learning of the present invention has the advantages that the chromosome karyotype analysis efficiency can be effectively improved, the recognition sequencing time can be shortened, automatic classification and sequencing of chromosomes can be completely with high accuracy.