Invention Grant
- Patent Title: Compositions, methods, and kits for one-step digestion of nucleic acid for analysis by liquid chromatography tandem mass spectrometry
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Application No.: US16758473Application Date: 2018-10-26
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Publication No.: US11441170B2Publication Date: 2022-09-13
- Inventor: Robert Ross , Patrick A. Limbach , Balasubrahmanyam Addepalli , Manasses Jora
- Applicant: UNIVERSITY OF CINCINNATI
- Applicant Address: US OH Cincinnati
- Assignee: UNIVERSITY OF CINCINNATI
- Current Assignee: UNIVERSITY OF CINCINNATI
- Current Assignee Address: US OH Cincinnati
- Agency: Dinsmore & Shohl LLP
- International Application: PCT/US2018/057696 WO 20181026
- International Announcement: WO2019/084394 WO 20190502
- Main IPC: C12Q1/6806
- IPC: C12Q1/6806 ; C12N9/16 ; C12N9/22 ; C12Q1/6872
Abstract:
A kit for preparing a nucleic acid sample for analysis by liquid chromatography tandem mass spectrometry (LC-MS/MS) is provided, the kit comprising: a lyophilized enzyme composition comprising: micrococcal nuclease; nuclease P1; and bacterial alkaline phosphatase (BAP); and a digestion buffer. Also provided are enzyme compositions and methods of use for rapid, efficient preparation of a nucleic acid sample for analysis by LC-MS/MS, without the need for denaturation of the sample.
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