A driving method for a backlight is provided. The backlight includes a plurality of light-emitting elements, and the driving method includes steps of calculating a brightness difference value of an image to be displayed; and determining whether the brightness difference value of the image to be displayed is greater than a predetermined difference value of the image. When the brightness difference value of the image to be displayed is determined to be greater than the predetermined difference value of the image, the method includes steps of dividing the image to be displayed into a plurality of sub-regions; partitioning the backlight according to brightness difference values of the plurality of sub-regions to obtain a plurality of final light-emitting regions of the backlight; and driving the backlight to emit light according to the plurality of final light-emitting regions. The present disclosure further provides a driving circuit, a backlight and a display device.