Provided is a method of manufacturing a heat radiation sheet. The method includes the steps of: electrospinning a first spinning solution comprising an adhesive, first heat conductive particles, and a first solvent to form an adhesive layer in a form of a nano-web having a plurality of pores; mixing second heat conductive particles and a second solvent to obtain a second spinning solution; electrospinning the second spinning solution onto the adhesive layer to form an intermediate layer; mixing a polymer material, a third solvent, and third heat conductive particles to obtain a third spinning solution; and electrospinning the third spinning solution onto the intermediate layer to form a heat radiation layer in a form of a web on the intermediate layer.