- Patent Title: Anti-VEGF protein compositions and methods for producing the same
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Application No.: US17500735Application Date: 2021-10-13
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Publication No.: US11459373B2Publication Date: 2022-10-04
- Inventor: Andrew Tustian , Ankit Vartak , Thomas Daly , Erica Pyles , Nisha Palackal , Shunhai Wang , Ning Li
- Applicant: Regeneron Pharmaceuticals, Inc.
- Applicant Address: US NY Tarrytown
- Assignee: Regeneron Pharmaceuticals, Inc.
- Current Assignee: Regeneron Pharmaceuticals, Inc.
- Current Assignee Address: US NY Tarrytown
- Agency: Kramer Levin Naftalis & Frankel LLP
- Agent Stephen J. Gaudet
- Main IPC: C07K1/36
- IPC: C07K1/36 ; C07K14/71 ; C12P21/06 ; C07K1/113 ; C07K1/16 ; C12N5/00 ; C07K1/18 ; C07K1/22 ; C12P21/02 ; A61K38/18 ; C07K16/28 ; C12N15/66 ; C07K16/22 ; G01N30/80 ; G01N30/02
Abstract:
The present disclosure pertains to compositions comprising aflibercept and methods for producing such compositions in chemically defined media and using anion exchange chromatography to produce aflibercept with a specific glycosylation profile. In particular, these methods produce aflibercept with a glycosylation profile with specific ranges of % total fucosylated glycans, total sialylated glycans, mannose-5, and galactosylated glycans.
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