Invention Grant
- Patent Title: Transglycosylation of endo-S and endo-S mutants for antibody glycosylation remodeling
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Application No.: US16022978Application Date: 2018-06-29
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Publication No.: US11459380B2Publication Date: 2022-10-04
- Inventor: Lai-Xi Wang , Xin Tong , Tiezheng Li
- Applicant: UNIVERSITY OF MARYLAND, COLLEGE PARK
- Applicant Address: US MD Waldorf
- Assignee: UNIVERSITY OF MARYLAND, COLLEGE PARK
- Current Assignee: UNIVERSITY OF MARYLAND, COLLEGE PARK
- Current Assignee Address: US MD Waldorf
- Agency: Carter, Deluca & Farrell LLP
- Agent George Likourezos
- Main IPC: C07K16/18
- IPC: C07K16/18 ; C07K1/04 ; C07K1/36 ; C07K1/02 ; C07K1/107 ; A61K47/61 ; C12N1/20 ; A61K47/54 ; A61K47/68 ; C07K16/28 ; C07K16/32 ; C12N9/24 ; C12P21/00 ; C12R1/46 ; A61K9/00
Abstract:
The present invention provides for a one-pot enzymatic approach which does not require removal of the enzyme and purification of the intermediate after deglycosylation step, and the Endo-S treatment is able to do both deglycosylation and transglycosylation. The one-pot strategy of the present invention enables chemoenzymatic synthesis of an azido-tagged N-glycoform of monocloncal antibodies which could be further modified through orthogonal chemical ligation for various applications.
Public/Granted literature
- US20190002542A1 TRANSGLYCOSYLATION OF ENDO-S AND ENDO-S MUTANTS FOR ANTIBODY GLYCOSYLATION REMODELING Public/Granted day:2019-01-03
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