A pixel array substrate includes a substrate, a plurality of data lines, a plurality of scan lines, a plurality of sub-pixels, and a first and a second auxiliary lines. The plurality of sub-pixels are arranged into first rows arranged in a first direction and second rows arranged in a second direction. The first auxiliary line and the plurality of scan lines belong to a first conductive layer. The second auxiliary line and the plurality of data lines belong to a second conductive layer. The first auxiliary line is located between two scan lines. A first end of the first auxiliary line is connected to one of the two scan lines. A second end of the first auxiliary line is separated from the other one of the two scan lines. The second auxiliary line is electrically connected to the first auxiliary line at the second end through a conductive via.