Invention Grant
- Patent Title: Sequence conversion and signal amplifier DNA cascade reactions and detection methods using same
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Application No.: US16816887Application Date: 2020-03-12
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Publication No.: US11492658B2Publication Date: 2022-11-08
- Inventor: Makoto Komori , Toru Yoshimura
- Applicant: ABBOTT LABORATORIES
- Applicant Address: US IL Abbott Park
- Assignee: ABBOTT LABORATORIES
- Current Assignee: ABBOTT LABORATORIES
- Current Assignee Address: US IL Abbott Park
- Agency: McAndrews, Held & Malloy, Ltd.
- Agent Audrey L. Bartnicki; Christopher P. Singer
- Main IPC: C12Q1/682
- IPC: C12Q1/682 ; C12Q1/6844 ; C12Q1/6886

Abstract:
Disclosed are methods for detecting a target nucleic acid in a sample. The methods include contacting the sample, in the presence of a polymerase and an endonuclease, with a first oligonucleotide comprising, in the 5′ to 3′ direction, a first signal DNA generation sequence, an endonuclease recognition site, and a sequence complementary to the 3′ end of a target nucleic acid; a second oligonucleotide comprising, in the 5′ to 3′ direction, a second signal DNA generation sequence, an endonuclease recognition site, and a sequence that is homologous to the first signal DNA generation sequence of the first oligonucleotide; a third oligonucleotide comprising, in the 5′ to 3′ direction, a third signal DNA generation sequence, an endonuclease recognition site, and a sequence that is homologous to the second signal DNA generation sequence of the second oligonucleotide.
Public/Granted literature
- US20200283822A1 SEQUENCE CONVERSION AND SIGNAL AMPLIFIER DNA CASCADE REACTIONS AND DETECTION METHODS USING SAME Public/Granted day:2020-09-10
Information query
IPC分类: